Hello,
In part of my project, i have to extract cell nucleus after cells lysis in order to measure nuclear radioactivity and also cytoplasm+membrane fraction radioactivity.
For this, it is important to check if my cytoplasm+membrane fraction and nuclear fraction are pure.
Would you have a check technic to advise me on how to do it ?
Thank you
https://www.thermofisher.com/ca/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-cell-lysis-and-protein-extraction.html
Subcellular fractionation can be done using a series of specific extraction buffers (e.g., for nuclear, cytoplasmic, membrane): typically within 2 hours when commercially available kits are used. “Purity” of yielded fractions can be checked, e.g., by western blotting for compartment specific marker proteins.
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