Cross-country research: How can I control for the factor country, although I don't have any hypotheses regarding country differences?

Hello, I am currently preparing a preregistered report for experiments in different countries. I am studying motivations/values of students; therefore, I want to conduct several same experiments...

28 January 2021 5,763 3 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View

How to desinfect a liquid nitrogen tank?

Hey everyone, we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside. The tank seems to be made of aluminum. Any recommendations on what disinfectant to...

07 January 2021 1,599 3 View

DIANA FEA 10.2: How do I model deformation supports, so that no tension occurs/is allowed ?

Hello together, I am working on concrete direct shear Tests, where i apply loadsteps of a prescribed Deformation (left deformation). Since i can only apply a Deformation with a support inplace i...

20 January 2020 8,225 3 View

Is HSE06 a good functional?

I'm investigating first row transition metal oxides on diamond surface, optimizing structures and calculating electronic properties (e.g. electron affinity). I was advised that since I'm working...

08 January 2020 8,713 4 View

Quick ('on the Fly') update of MM potentials, MD software with Amber ForceField?

Hello, Could anyone recommend a free MD software (that takes Amber ForceFields) where one can very quickly (on the Fly) update the MM potentials of a set of atoms (and query the coordinates of...

20 November 2019 7,716 1 View

What test shall I use when I want to compare an experimental group at various time points to one control group?

The data are as follows (overview of the design attached): One parameter was measured in the blood of the same patients at various time points and also in a healthy control group at one time point...

15 July 2019 1,382 6 View

Can I use ELISA with not ideal standard curve?

Dear colleagues, I would like to ask you if you have any experience how to use data from ELISA analysis when my standard curve is not as showed in the manual? I have many standard curves that have...

17 April 2019 282 4 View

Does it exist any open repositories of user stories?

For a Machine Learning-based project, I'm looking for repositories containing user stories (and acceptance criteria). This can be issue management systems (e.g., stories in Jira) or plain...

19 December 2018 4,195 7 View

What are possible portable multigas sensing devices?

Hi, I'm currently looking for a portable gas measurement system to measure O3, NO2 and CO at the same time. I already stumbled upon GrayWolf Sense and Aeroqual. Can someone suggest other...

13 September 2018 6,690 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View