How are ways to communicate with students in the classroom to find different ways of communicating in e-learning?

18 October 2020 4,749 3 View

How to make boxplot in R or anyother app?

Hi Any help is appreciated. How to produce a boxplot graph as in the attached image (from Gregory et al., 2019)? using R or any other software?

15 December 2019 2,208 4 View

Can anyone help me find an Instrument to measure rogers five attributes of innovation?

I am currently researching voluntary reporting and its determinants through the (DIO) theory of innovation diffusion. I need a measurement tool other than interviews such as questionnaires if...

02 December 2019 6,128 4 View

Who can help me.I need papers on the Fuzzy Activity Based costing system ?

Fuzzy cost systems generally aim to counter uncertainty in estimating cost vectors as inputs to costing systems, making cost estimates accurate, as well as increasing reliability in results. These...

08 November 2019 9,679 1 View

What do you think about distance study? Do you expect it to succeed in developing countries under the circumstances it is going through?

10 October 2019 8,101 14 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

03 July 2019 9,461 3 View

A tool for creating synthetic time series based on the existing one?

Hello, I have a historical time series of 72-year monthly inflows. I need to generate, say 100, synthetic scenarios using the historical data. Simple resampling (by reordering annual blocks of...

26 April 2018 4,512 6 View

Can anyone tell me how to use FASTA sequence protein to visualise its structure?

Visualising protein structure

07 October 2013 2,339 14 View

Any suggestion for PHD proposal in health sector

Good morning I hope you all doing great and safe I got Bachelor degree on health administration and master on health administration concentration in health informatics . I want to start a PHD...

01 January 1970 866 3 View

How could find the perfect target for miRNAs?

Dear Sir/Madam I know there are some websites giving you the target predicted for microRNA. I am a little confused about them. Can anyone has a reliable websites that is helpful to get the best...

01 January 1970 1,404 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View