Dear Sir. Concerning your issue about the test of cytotoxicity of chitosan nanoparticles in ZFL cells. Nanoparticles are increasingly being developed for in vivo use, from targeted drug delivery to diagnostics, where they have enormous potential, while they are also being used for a variety of applications that can result in environmental exposure for humans. Understanding how specific nanoparticles interact with cells and cell systems is essential to gauge their safety with respect to either clinical or environmental exposure. Zebrafish is being increasingly employed as a model to evaluate nanoparticle biocompatibility. This review describes this model and how it can be used to assess nanoparticle toxicity at multiple levels, including mortality, teratogenicity, immunotoxicity, genotoxicity, as well as alterations in reproduction, behavior and a range of other physiological readouts. This review also provides an overview of studies using this model to assess the toxicity of metal, metal oxide and carbon-based nanoparticles. It is anticipated that this information will inform research aimed at developing biocompatible nanoparticles for a range of uses. I think the following below links may help you in your analysis:

https://www.researchgate.net/publication/326575385_Zebrafish_as_a_Model_to_Evaluate_Nanoparticle_Toxicity

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260029/pdf/ijn-6-3351.pdf

https://ac.els-cdn.com/S1021949814001410/1-s2.0-S1021949814001410-main.pdf?_tid=4d5e6b22-0ced-4785-96a9-3b4acc15ee77&acdnat=1539206769_b66bfebaba2a7eb43dfccd2cd949ebde

Thanks

Dear Sir.

It is definitely possible. You can study below article:

Article Synthesis and in vitro study of modified chitosan-polycaprol...

Isam Eldin Hussein Elgailani and Asghar Narmani

Thanks for your collaborations. These publications certainly will improve my knowledge about this theme.