Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

Heat flux and heat losses on a thermal conductivity measuring device?

Hello, I am currently working on the design of a device that measures thermal conductivity of sediments and I am not sure if i estimated heat losses and heat flux in the correct way. The device is...

23 September 2020 8,119 6 View

Will exercise/physical activity in remote mode become a trend due to COVID-19 outbreak? any available evidences?

Given that fitness centers and other training locations are temporarily closed, it is possible to see some opportunities for physical activity and health promotion and a way to delivery and...

01 June 2020 9,294 13 View

How to fix SPSS PROCESS macro access issue in macOS Catalina?

Im trying to install PROCESS tool on SPSS in a Mac but it doesn't work. Anyone facing the same issue. any solutions? Tks

31 May 2020 483 2 View

Can anyone recommend a good software that helps keep track of advances in different academic projects, both in group and individually?

I am currently working on many different research fronts at the same time. I would like to keep track of my advances and relate tasks when various projects --eventually-- overlap. I am currently...

01 April 2020 824 3 View

How to plot dose-response from RT-qPCR data?

I've performed experiments to analyze the effect of different concentrations of a drug on gene expression using RT-qPCR. It is not clear to me whether data should be ploted as Fold Change vs....

22 March 2020 1,912 5 View

Hello everyone, could you tell me the structure of Alexa Fluor 680 ??

16 October 2019 1,573 4 View

Does anyone work with femoral and tibia radiography on rats or mices?

I would like to know what protocol followed for animals undergoing some treatment that induces some type of osteopenia / loss of bone density.

13 July 2019 1,731 2 View

Georreferencing .tif images on Matlab?

I need to georreference a .tif file containing a single band image with pixel depth 16 bits (unsigned). The number of columns and rows is 946 and 843, respectively. The boundaries of the image are...

01 May 2019 2,409 8 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View