Can I run a sample on an agarose gel, refrigerate it, and run it again at another time?

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Diana Sue Smith

If your bands are small, diffusion will cause the bands to spread and decrease the visibility. This is a function of both band size and % agarose as well as temperature. Refrigeration helps with that but don't leave gels with small bands too long. A couple of days would not be a good idea in that case. Also make sure your gel remains hydrated in the running buffer with EDTA.

Dejun Li

Yes, you'd better wrap your gel with preservative film and put it in refrigerate. When you have time to work again, you remove the preservative film and restart your experiment.