Is NHS soluble in dichloromethane?

I am trying to dissolve 0.1 g of N-Hydroxysuccinimide in about 5 mL of dichloromethane in a round bottom flask with a magnetic stirrer. I cannot get the NHS to dissolve unless I add 30 mL of...

19 November 2020 9,152 1 View

Primer works for PCR but not for Sequencing?

I'm doing an LR reaction to get my gene from a pENTR vector to a pDest vector. After the transforming the LR reaction, my negative control (just the pDest vector, so all cells should be dead from...

25 January 2017 8,623 8 View

How can I resuspend a Glass milk solution for geneclean that dried up?

I'm using the geneclean kit that uses glass milk to separate DNA. I've used it previously, but it's been a few months and the glass milk is dried up. I've vortexed it and heated it, but can't get...

19 November 2015 5,503 1 View

Can agar be used instead of agarose to run an electrophoresis gel?

Can agar be used instead of agarose to run an electrophoresis gel? Or will the bands not be defined enough?

01 April 2015 4,683 7 View

Wearable technology and sustainability -reading recommendations?

Hello there! I'm working as an RA in a project that examines wearable technologies (production and consumption) from a sustainability perspective. Do you have some reading suggestions, journal...

01 January 1970 2,087 5 View

Responsibilities of covert researchers

I would like to know if there is any consensus or further reading available with regard to the responsibilities of researchers who are covertly observing members of the public. For example: a...

01 January 1970 7,148 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

No title

02 March 2021 3,060 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View