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Questions related from Jenna Labelle
I'm doing an LR reaction to get my gene from a pENTR vector to a pDest vector. After the transforming the LR reaction, my negative control (just the pDest vector, so all cells should be dead from...
26 January 2017 8,674 8 View
I'm doing an LR reaction (gateway cloning) to move my gene from a pENTR (kan resistance) vector into the Dest8 (amp resistance) vector. After performing the reaction according to the...
06 January 2017 8,574 6 View
I'm using the geneclean kit that uses glass milk to separate DNA. I've used it previously, but it's been a few months and the glass milk is dried up. I've vortexed it and heated it, but can't get...
20 November 2015 5,548 1 View
Can agar be used instead of agarose to run an electrophoresis gel? Or will the bands not be defined enough?
02 April 2015 4,758 7 View
Something I've always wondered about- if I'm running a electrophoresis agarose gel and something comes up and I cannot finish it, is it possible to refrigerate it as per usual, take it out of the...
27 March 2015 7,584 2 View