I would like to know if is possible to re amplify and PCR fragment for Gibson Assembly ?
I did cut and purified the gel band but the amount in ng/uL is not enough for Gibson Assembly later.
Dear all, I have recently come across several examples in Social Sciences where supervisors become co-authors of published articles by MSc and PhD students. While I understand a supervisor might...
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Is there a simple formulation to estimate the composition and amount of salt precipitation (in equilibrium) given the ion concentrations in a brine and the temperature conditions? For example: if...
01 February 2014 9,206 2 View
I have used sequences from transcriptomics to design primers for RT-PCR. The gene was found over-expressed in specimens with high infection, thus I expect to confirm the result by RT-PCR. However,...
25 October 2012 5,788 16 View
Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...
17 February 2021 2,896 3 View
I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...
16 February 2021 8,872 2 View
Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...
14 February 2021 2,310 1 View
I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...
08 February 2021 8,731 2 View
The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...
05 February 2021 3,459 2 View
Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...
03 February 2021 2,032 3 View
Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...
03 February 2021 6,379 3 View
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...
31 January 2021 9,495 2 View
Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...
29 January 2021 1,406 2 View