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Questions related from Onias Castellanos Collazo
I need to evaluate viral accumulation by qPCR in plants with DNA and RNA virus coinfections . I dont know how proceed in tge sample treatment before the qPCR . Please someone has done this before ?
05 December 2023 3,628 0 View
Im running a Gibson Assembly, using two fragments of 2750 pb and a vector of 3300pb. Then when I transform in DH5alfa or 10 beta got a higher band than I expected. Also I did PCR from miniprep and...
04 December 2022 3,335 0 View
I would like to know if is possible to re amplify and PCR fragment for Gibson Assembly ? I did cut and purified the gel band but the amount in ng/uL is not enough for Gibson Assembly later.
23 September 2022 2,082 0 View
I'm designing primers for Gibson Assembly . My main query about that is the harpain and self dimer formation ? Did you have problems with that ?
21 January 2022 2,704 5 View
I need to get a dimeric infective clone from a Capulavirus, a Geminivirus of simple strand DNA. So I would like know some conditions for the procedure of obtaining it. I´m using XhoI which only...
01 January 1970 9,857 2 View
This is my second Gibson Assembly mix. The third well is the Nebuilder Positive Control and the fifth well is my Gibson Assembly mix. I have done tw fragments of 2.7 kb and the vector which is 3.3...
01 January 1970 9,631 3 View
