Hi All,
As my problem, I am working with ES cell lines and want to induce them into neurons. in this process we want to observe it as GFP+ cells. but seems the promoter (i.e ef1a) express high in es cell didn't working well in neurons, and neuron specific promoter didn't working in ES cells.
So I was thinking make a plasmid, has 2 promoter. like this:
neuron promoter-es cell promoter-GFP-polyA.
So that in es cell stage, the es cell promoter can working, and in neurons, the neuron specific promoter can working. but I didn't see any plasmid like this.
Does anyone do this before?
Many thanks
Yiyun
I have never tried that, however it is a low possibility that both will function.
May be you should try to make a plasmid like this.
neuron promoter-GFP- es cell promoter-GFP-polyA
Ok seems all of reply said need two promoter with two protein, and Paritosh's idea is great, so that I can see the process from ES cell to neurons. Thanks all of yours!
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