14 Questions 13 Answers 0 Followers
Questions related from Monica Yiyun Jiang
Hi All, I'd like to inhibit sox2 express or down regulated in my current cell culture, but shRNA method seems hard to apply as my cell line is a specifial neuron pregenitor and very hard for...
27 July 2016 6,623 1 View
Hi All, I have question about colocalization analysis for 3 channels. I attached an image to make my questions more easily understand. I have 3 images by confocol, from 3 channeal, green, red and...
19 July 2016 9,240 6 View
Hi All, Recently our lab want to delivery plasmids into primary cultured CNS neurons.I have tried lentivirus, lipo transfection before, and now we want to try electroporation. I checked online...
29 March 2016 5,026 3 View
Hi everyone, I extract total RNA for my sample (mouse cell culture) then convert to cDNA and PCR, concentration and A260/280 is 300 ug/ml and 1.97, I feel it quite normal, but no bands even for...
04 January 2016 4,999 16 View
Hi everyone, I really get a weired problem, I used a plasmid transfection cells, the plasmid has a GFP reporter linked with a protein (lets call it A protein) which I was interested, after...
24 November 2015 524 4 View
Hi All, I'm trying to infection brain primary culture by lentivirus but it seems all i got was glia, I heard that some chemicals can 'kill' all glias and leave almost pure neuron culture, I google...
18 November 2015 6,269 5 View
Hi All, Recently I want to study neurogenesis, and try to use Edu as a cell marker for proliferation. However the edu not working very well so I decided change it to other markers, such as Ki67 or...
03 August 2015 452 5 View
Hi All, Recently I generate a vector (~6k bp, amp resistence) which only have EcoR1 and EcoR5 in MCS and can let me insert cDNA between this 2 enzyme. and This vector has been sent to sequencing,...
04 May 2015 4,456 4 View
Hi All, As my problem, I am working with ES cell lines and want to induce them into neurons. in this process we want to observe it as GFP+ cells. but seems the promoter (i.e ef1a) express high in...
16 April 2015 8,965 3 View
Hi All, I meet a ligation problem. I digest my vector with EcoR1/EcoR5, and insert with Mfe1/EcoR5, after ligation and heatshock, there is no colony grow. I run a gel with my ligation products, it...
02 April 2015 9,404 3 View
Hi All, recently I was thinking using SYN promoter instead of my CAG promoter to get better GFP express in neuron. Then I searched, I found SYN promoter seq is little bit different between...
25 March 2015 5,337 2 View
Hi All, Recently I'm working on an AAV plasmid and trying to make new plasmid. And I checked on many website, some said to use stbl3 and some said use stbl2. I got confused about it and didn't see...
03 December 2014 2,232 2 View
Hi All, Recently I tried to make a GFP-ES cell line which can express GFP strongly. I used pCDH Vector(PKG-GFP) and packaging lentiVirus to transduce my ES cell. However, the GFP is so weak so I...
24 November 2014 6,105 2 View
Hi everybody, Recently I move on to a new projects about circRNA and a disease, very interesting. My boss got a company to sequencing every published circRNA with patient's samples, around 1000...
01 January 1970 4,745 3 View
