Omer Faruk Kuzu

4 Questions 18 Answers 0 Followers

Questions related from Omer Faruk Kuzu

Has anyone tried to stably or inducibly over-express myogenic genes (eg, myogenin, MyoD) in C2C12 cells?

I know that it is possible to induce differentiation of C2C12 cells via serum deprivation. And there is a very good blog about it in here:...

03 January 2018 1,963 0 View

Expression of which genes can be used as markers of various cellular abnormalities such as ER stress, oxidative stress, autophagy?

Hi, I am interested in using q-RT-PCR to screen abnormal cellular processes such as ER stress, oxidative stress, autophagy, mitophagy, abnormal Golgi function, abnormal intracellular...

07 January 2017 7,689 5 View

Is this a Caspase dependent or independent cell death?

Is there any apoptosome dependent but caspase-independent cell death pathways? I have a compound that triggers cell death. Co-treatment of 20 uM zVAD fmk fails to inhibit cell death suggesting...

21 May 2014 5,830 17 View

Problem regarding the stable transfection of floxed GFP into a B16F10 mouse melanoma cell line.

I am trying to obtain a stably transfected B16F10 cell line of floxed GFP plasmid (http://www.addgene.org/8389/). The plasmid structure is pCMV-lox-GFP-lox-RFP. Thus it is expected to express GFP...

13 November 2012 541 9 View