We've got a problem with OD600 after overnight growth in bioreactor namely after
12 h we've reached 8 ,
7 and 6 during three day respectively . We want to establish DO cascade by means of glucose dosage by pump , but maybe this is wrong solution we've no enoug experience in this subject ( maybe 'll be better we have to set up ph in cascade instead of DO )
Thank you for your suggestions
Do you mean when you see a DO spike you turn on the glucose pump? that could work, but you also need to make sure that the DO is high enough that it won't impede the bacteria growth. otherwise DO is usually controlled with gas flow rate and agitation speed, and possibly the use of pure oxygen. The following article has more detailed information:
https://plygenind.com/how-to-control-dissolved-oxygen-do-in-bioreactor/
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