We've got a problem with OD600 after overnight growth in bioreactor namely after
12 h we've reached 8 ,
7 and 6 during three day respectively . We want to establish DO cascade by means of glucose dosage by pump , but maybe this is wrong solution we've no enough experience in this subject ( maybe 'll be better we have to set up pH in cascade instead of DO )
Thank you for your suggestions
is your DO cascade linked to nutrition pump? or just gas flow, propeller etc.? if your OD600 was decreasing, that means there are some restriction factors in the cell growth, if DO is not the restricting factor, it could be carbon source, nitrogen source, or pH etc. You need to first find out this restricting factor, and then adjust this during the fermentation run. https://plygenind.com/how-to-control-dissolved-oxygen-do-in-bioreactor/
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