Piotr Bąska

6 Questions 5 Answers 0 Followers

Questions related from Piotr Bąska

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

18 February 2021 9,172 4 View

Which betaine is the best for LAMP?

I want to perform LAMP analysis. According to the procedure betaine should be added. Anyone knows the best betaine for LAMP. I found three from Sigma aldrich. Betaine (Can no.61962) costs less...

06 February 2015 2,302 3 View

Is it possible for HTERT and SV40 cells to be immortalized using the same promoter?

I want to immortalize cells using hTERT and SV40 using vectors: pLXSN/SV40, the SV40 would be expressed from 5`LTR promoter, the plasmidgives resistance for neomycin, pMSCVhyg/hTERT, the hTERT...

09 January 2015 7,842 1 View

How can I identify a gene promoter (Cis-regulatory element)?

I want to check if promoters of a few genes (from cattle) are active in the cells. I want to clone GFP under the promoters and transfect the cells with the vector. The problem is I don`t know the...

20 December 2014 6,012 3 View

Does anyone know how to calculate the exact amount of RNA in the sample where 260:280 and 260:230 are not perfect?

Let's say 1,6 and 1,5 respectively. I want to know how much RNA there is in the sample. Is there any equation to eliminate protein and phenol influence on RNA concentration calculated by NanoDrop?...

20 March 2014 3,731 3 View

Can I make a coculture of epithelial cells of one strain and dendritic cells of other strain?

Will there be any non physiological interaction?

19 November 2013 6,352 3 View