I want to find encapsulation efficiency of drug in liposome.
Can I separate non-encapsulated drug by dialysis and calculate E.E from them?
I use DSPC as phospholipid and N-acetylcysteine which is hydrophilic as a drug.
It's really better use your liposome after dialysis for your purpose.
The way you said is possible too but no accuracy.
Regards.
yes you can ,Firstly, place a certain amount of liposome inside the dialysis bag, then place the bag in your buffer with stirring and then take samples from the donor medium (liposome inside bag) and the receiving medium (buffer) at specified intervals .after disposing of the free drug you can collect your samples and inject them on HPLC to determine the encapsulation efficiency of your drug in compare with original concentration of drug
regarding the liposomes samples (inside bag), you should be destruction of liposome before injection on HPLC , then, take the supernatant( after centrifugation and sonication ) and inject it
hope my answer will be useful for you
I agree with the previous answers. If your liposomes pellet during centrifugation, this would be an easier way to separate them from unencapsulated compound though. Another possibility would be ultrafiltration, e.g. using Amicon centrifugal filters.
You can use a size exclusion column to safely and efficiently separate your liposome and unencapsulated drug. This is the best way to keep liposomes intact. Afterward, you can just lyse the liposomes and run whatever test you need to quantify the drug.
We used dialysis method for removing non-encapsulated drug in our nanobubbles through dialysis. You can find the methods and details here. The composition of our nanobubbles contain phospholipids, so the process would be similar in your case.
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