Peter Yang

2 Questions 22 Answers 0 Followers

Questions related from Peter Yang

Can anyone help with lectin purification using strong base?

Hi, We are trying to express and purify a bacterial lectin (mannan specific) in E. coli. After sonification, we would like to apply the crude extract to a mannan-agarose (Sigma Aldrich) column....

01 June 2015 2,240 2 View

Why does bovine serum albumin prevent negative control from auto-agglutination in Hemagglutination Assay?

I'm currently expressing and purifying a lectin in E. coli BL21. To test whether the lectin has been successfully purified, we did a hemagglutination assay. The lectin is supposed to be a positive...

02 February 2015 9,722 4 View