How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

How to do qualitative field research in a hostile environment?

It is much said about doing qualitative research and field study. I'm curious about your hints/experiences in doing ethnography in hostile environment touching sensitive topics (marginalized...

30 November 2020 4,151 14 View

What is the scenario for the gold price?

What do you think about the further growth of gold price? Do you think that the upward trend is coming to an end? Or may be we observe a temporary turbulences caused by the US presidential...

23 September 2020 1,344 4 View

How to make a one-dimensional SOM (Kohonen) map?

Could you please help me and give some tips on how to design trajectory/line using a one-dimensional Kohonen "map"? I need it to my article on bankruptcy but as I'm not statistician I can't handle...

23 September 2020 6,834 2 View

What's the acceptable value of _refine_ls_shift/su_max for Rietveld refinement?

Hi, my background is in single-crystal X-ray diffraction. Now, I am carrying out the so-called Rietveld refinements in GSAS-II and am getting quite large _refine_ls_shift/su_max values (ranging...

01 September 2020 7,012 2 View

How can I start a quantum chemistry calculations with Gaussian with modified basis set?

I want to run geometry optimization in Gausian. Currently I am running it this way: #N B3LYP/3-21G OPT NOSYMMETRY 0 6 Dy 0.00000000 0.00000000 0.00000000 F 2.14203091 0.10643632...

27 June 2020 2,912 9 View

A queueing model for the cloud computing centre?

I'm considering an analytical model for the cloud computing composed of: - input load balancer (LB) with M/M/1/K - P (PH) physical machines with hypervisors, each able to host V (VM) virtual...

18 November 2019 2,356 3 View

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07 November 2019 2,121 1 View

Suitable PhiX concentration for diversified libraries (MiSeq v2, Nextera XT)?

Dear All, could some please suggest proper PhiX concentration for multiplex Illumina sequencing with use of MiSeq kit v2 and Nextera XT. samples #1-11 are highly similar - around 50-56%GC sample...

07 September 2019 2,793 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View