Recently I bought this from Thermofisher. After performing the RNA isolation according the manufacturer's protocol, I got the bands like this. Could you please clarify me if this is Total RNA or not?
Thank you!
https://www.thermofisher.com/order/catalog/product/AM1560?SID=srch-hj-am1560#/AM1560?SID=srch-hj-am1560
Kanaka Sai Ram Padam I did not see a band. And, where is size markers?
Hi Kanaka, yes you can, as this kit is designed to extract both the total and smaller RNAs. As far as, you get most of the aqueous organic phase carefully without contaminating with lower interphase, and then proceed to total RNA protocol, it should be fine. Take care about the temperature of absolute ethanol, as it must be maintained at room temperature, also use fresh new bottle of ethanol as it is being very hygroscopic hence gets diluted upon log term storage. mirVANA PARIS kit is not compatible with spleen and pancreas tissues as per the manufacturer guidelines. Never spin filter cartridges above 10000 g, it seriously may damage filters, which I have personally observed. Preheating of elution solution at 95 C is very critical step in PARIS kits, so take a good care of it. If you have Qubit fluorometer so measure your RNA via HS or BR Qubit RNA quantification kits, as these will give you more authentic quantification of your RNA than the nanodrop, and while quantifying RNA with nanodrop focus well on your 260/230 ratios, which should be at or above 1.7 for all of your RNA samples.
Regards....
This kit is doing a great job at purifying total RNA- long and short, through miRNA fraction ~ 20 nt
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