Can someone please tell me if this is DNA or RNA?

More Kanaka Sai Ram Padam's questions See All

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Please help me out with this question. Does the assumption of normality need to be met for a mediation analysis in PROCESS in SPSS?

Does the assumption of normality need to be met for a mediation analysis in PROCESS MACRO in SPSS?

06 January 2021 2,195 6 View

Can cultured cells be stored at -80DegC?

Can someone please answer me this. I am storing the cells dissolving it in the freezing media(culture media + 5% DMSO) at -80 deg C. Is it safe to do?.

02 April 2020 4,648 3 View

How can one separate bare soil, built-up area, and river sand in Landsat TM and OLI images?

I am trying to make LULC of an area to access temporal changes using Landsat imageries of TM and OLI sensors. The problem is that the bare area, sand in river body, and built-up area appear same....

11 March 2020 6,733 3 View

How do I culture a cell line from Tumour tissue?. A primary cell line?

I have been trying to culture cells from the tumour tissue sample I obtained. I homogenized the tissue sample and then seeded the sample into culture medium. But no result or there was a...

21 February 2020 4,406 4 View

I need to calculate pKa for an acid and I don't know which output of freq gave me gibbs free energy?

I found in gaussian that gibbs free energy is sum of the total electronic energy and correction to the gibbs free energy but in some articles its sum of just gibbs free energy in the output of freq.

05 May 2014 5,643 8 View

I want to calculate proton affinity for an acid, how can I add hydrogen?

After adding hydrogen,the acid separated.

04 May 2014 9,137 4 View

I have negative frequency for my optimization structures. What should I do?

I reoptimized after moving atoms but don't use opt=tight because of cpu time

27 April 2014 7,432 6 View

In the unrestricted B3LYP optimization, which MO is better to use? Alpha or beta?

Someone said that change the unrestricted keyword to restricted in the output of MOs,but when i do that it select just alpha MOs, I don't know if it is the correct way and when compared with beta...

27 April 2014 9,927 10 View

How can we measure teacher effectiveness?

Teacher effectivness is the measure part of quality education. Efficient and effective teachers performance is different than normal. But the main problem lies in assessing their work.

23 May 2012 1,860 93 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Manuel Balparda

Is not clear in the picture, but if you see two close bands is possible that you have RNA. A single band is DNA. Hope that you solve the problem!

Benyam Zenebe

This may be DNA although it is not clear in the picture. In the case of RNA you are expected to see two bands from the same sample for the two subunits of ribosomal RNAs: 18S and 28S RNAs and your mRNA would form a smear instead of a band.