Kanaka Sai Ram Padam

4 Questions 2 Answers 0 Followers

Questions related from Kanaka Sai Ram Padam

Can cultured cells be stored at -80DegC?

Can someone please answer me this. I am storing the cells dissolving it in the freezing media(culture media + 5% DMSO) at -80 deg C. Is it safe to do?.

03 April 2020 4,811 3 View

Can I isolate the Total RNA from mirVana miRNA Isolation Kit?

Recently I bought this from Thermofisher. After performing the RNA isolation according the manufacturer's protocol, I got the bands like this. Could you please clarify me if this is Total RNA or...

28 March 2020 5,481 5 View

How do I culture a cell line from Tumour tissue?. A primary cell line?

I have been trying to culture cells from the tumour tissue sample I obtained. I homogenized the tissue sample and then seeded the sample into culture medium. But no result or there was a...

22 February 2020 4,590 4 View

Can someone please tell me if this is DNA or RNA?

I have isolated gDNA from cancer tissue and later run it on 0.8% Agarose Gel Electrophoresis for 30 mins at 50 volts. I got the bands as attached in the pic below. Can you please clarify me what...

05 February 2020 262 3 View