Can someone clarify me this.

I have isolated the total RNA and converted it into first strand cDNA using an appropriate kit. Now the converted first strand cDNA is no more similar to the target RNA but it is complementary to it, right?.

And I retrieved my target sequence (RefSeq) from NCBI. Now for Real Time PCR, do I have to design primers using the sequence I obtained from the NCBI RefSeq or do I have to take the complementary sequence of my target gene to design the primers for expression?.

I got confused around the first strand cDNA.