What is the RNA concentration in cell culture media?

The RNA concentration in serum according to literature is about 5-40 ng/ml. However, there is very little information about the RNA concentration in conditioned medium of cultured cells....

27 June 2019 6,364 7 View

Does MHC-II Ab clone M5/114.15.2 block the receptor?

Hi, I want to sort cells using clone M5/114.15.2 (MHC class II), and then use them in a T cell activation assay. Does anyone know if the bound antibody from sorting will block the MHC-II receptor...

10 April 2018 1,451 6 View

How calculate the dose of nano drugs as usually lower than the standard one?

why nano doses uaually lower than the standard drug doses depends on what or how can i calculate it accurately?

09 October 2017 2,082 3 View

How can I embed a snap frozen tissue in OCT?

I have an already frozen tissue, and I want to embed it in OCT in order to section it for immunostaining. What is the best way to do it without ruining the tissue structure and without subjecting...

17 August 2016 2,229 8 View

What will be the most efficient way to amplify your cDNA of choice for the purpose of over-expressing it later?

Should I use Oligo-dT with a high processivity reverse transcriptase to make the total mRNA and then use specific primers to PCR amplify it? or is there a better way? Thanks

14 January 2015 2,398 3 View

How can I measure empirically the use of linguistic expressions of self-agency? I am looking for validated paradigms for the research of self-agency.

Here are some examples of such linguistic expressions: active versus passive verbs, unaccusative verbs, nominialization, avoidance of personal pronouns etc.

10 July 2013 8,115 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View