can I get suggestions regarding kieselguhr cholesterol column packing for cholesterol oxidase protein purification?
link.springer.com/article/10.1023/A:1008958008333
For bioethanol production brewers yeast is used. But which commercial yeast is preferred for higher productivity? Thank you
11 May 2022 2,824 8 View
I performed a DSC analysis of rice starch samples to determine gelatinization enthalpy and peak temperature. I'm expecting a gelatinization temperature between 60 to 80 celsius. What could be the...
29 March 2022 7,352 16 View
I have grown a mixed culture of E.coli and lab (different strains) together in a microplate. I would now like to see if lab is alive and would like to enumerate on an agar plate which could only...
17 December 2021 8,148 3 View
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14 December 2021 9,926 4 View
Drug ethanolic extract of A.calamus. vehicle CMC.
14 December 2021 6,250 0 View
29 August 2021 6,130 6 View
When we isolate Bacteria or fungus from seeds how can we tell whether they are endophytes or pathogens....is there any method or parameter to differentiate them...can anyone help answering this?
05 August 2021 6,114 3 View
Hi, Can I please know if a bacterial culture is inoculated in 1 ml nutrient broth and incubated overnight in the centrifugation tubes? Will 1 ml of the nutrient broth be sufficient for the culture...
05 July 2021 3,306 2 View
Hi, I have a question, I inoculated the fresh bacterial culture and incubated overnight in 5ml nutrient broth, I must centrifuge 4430*G for 5 minutes followed by decantating the supernatent and...
20 June 2021 5,496 2 View
By taking any program point and a boolean variable, can we perform an abstract slice based on the boolean property.
13 June 2021 291 0 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View