I am preparing RNA later following the patented protocol. It is mentioned there and in other recipes as well to use H2SO4 to balance the pH to 5.2. If H2SO4 is not available, can I use HCL instead of H2SO4? Does it affect the RNA later potential?
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 179 3 View
how is prion can cause disease
01 August 2024 249 1 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.
31 July 2024 10,108 3 View
Please note it
29 July 2024 4,919 3 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/
29 July 2024 5,160 1 View
I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...
24 July 2024 1,514 0 View
If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.
19 July 2024 3,356 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.
10 August 2024 7,524 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,618 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,364 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 223 2 View