Is it possible to create an organoid containing stroma?

Hi, I work on mouse oviduct organoids and would like to study the stromal cells. Is it possible to create an organoid containing stroma? So far I have created organoids from mouse oviduct tissue...

04 August 2020 9,236 1 View

How many images does Imagedatagenerator generate (in deep learning)?

Can anybody explain to me this? Is there any formula or equation to predict manually, the number of images that can be generated.

19 April 2020 2,766 3 View

Can anyone address this Ansy system coupling error "Fluent threw an exception" ?

I have tried searching a lot and it keeps giving this particular error again and again. Note; The Ram and disk space are not an issue. The convergence has taken place. This error pops up once we...

17 January 2019 2,048 5 View

Why do HEK 293 cells detach as a sheet/layer after transfection?

I seeded the cells into a 12 well plate and transfected 1 ug of plasmid /well. For some reason, after 24 hours, I notice that in some wells, the cells have detached as a sheet. The entire layer of...

05 September 2017 4,034 12 View

What concentration of amino acids are required for bacterial growth in minimal media?

24 February 2016 5,534 3 View

How can we avoid agglomeration in nanoparticles?

30 March 2015 9,582 11 View

How we can isolate particularly pRET-B plasmid from the rosetta?

Master plate of DH5a is not working now, but I have that plasmid in rosetta that contains its own plasmid also. So how can I isolate and purify my own one?

07 May 2014 4,109 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View