Hi all,

I'm conjugating streptavidin to AuNPs. Protein solution is in 50mM phosphate buffer and mixed with AuNPs (also in PB). A protocol suggests that in the washing process, after centrifuge the pellet should be resuspended in 50mM Tris + 0.15mM NaCl.

Later in the blocking protocol, BSA is in PB and the pellet is resuspended in Tris also.

I'm wondering if there could be any issue with exchanging buffer of different kind.

Any comments would help.

Thank you.