Andrew Park

6 Questions 5 Answers 0 Followers

Questions related from Andrew Park

TNS assay for measuring pKa of a lipid nanoparticle system. Can I get some help?

Hi all, I'm setting up a protocol for TNS assay to measure pKa of a lipid nanoparticle I'm working on, and scratching my head trying to come up with buffer recipes. I'm primarily starting from...

29 February 2024 5,721 0 View

How to produce single-stranded DNA from LAMP PCR?

Hi all, I'm seeking ideas on producing single-stranded products from LAMP PCR. I think asymmetric PCR might work, but with no certainty. Any ideas or comments would help. Thank you.

26 December 2022 1,932 3 View

Can I exchange buffer for gold nanoparticle suspension (Phosphate buffer -> Tris HCl)??

Hi all, I'm conjugating streptavidin to AuNPs. Protein solution is in 50mM phosphate buffer and mixed with AuNPs (also in PB). A protocol suggests that in the washing process, after centrifuge...

16 September 2022 8,250 0 View

What can I use to buffer superacid?

Hi all, I'm using aqua regia to clean glassware used in synthesizing colloidal gold. When disposing aqua regia, one protocol suggested buffering with Sodium carbonate. I wonder if I can use any...

05 September 2022 8,924 0 View

How to block streptavidin-coated gold nanoparticles?

Dear all, I'm working on coating gold nanoparticles (AuNP) via EDC/NHS coupling and blocking them. And I came across a few hurdles that need your help. 1) Is there any precuations when...

22 August 2022 9,470 3 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

09 February 2021 9,220 5 View