The use of dyes like Coomassie blue or Eosin-Nigrosin to evaluate the sperm acrosomal status have a correlation with the fertility of bulls or stallions? Is it a good alternative to evaluate sperm under field conditions instead Fluorescence dyes like FITC-PNA/PI? What it's the best alternative to evaluate the acrosomal status, plasma membrane and viability of spermatozoon?

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