13 September 2022 0 6K Report

I am working with eutectic solvents to extract bioactive compounds and want to recover them, however, when trying to use the anti-solvent technique (water, 1:20), the solvent components do not separate.

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How to identify wetland area in Landsat imagery?

The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.

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Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

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Is there a way to calculate cell density and thus cell count from confluency for RPE-1 cells?

I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...

04 July 2024 9,622 0 View

Why am I seeing no color change of the positive control cell death assays?

I am currently running Cell Death ELISAs, using the Roche Cell Death Detection ELISA kit. My samples are from the supernatant of bacterially infected primary canine cells. I am seeing no color...

02 June 2024 8,682 1 View

Latest Advancements in Minimally Invasive Heart Surgery ?

What are the latest advancements in minimally invasive heart surgery techniques, and how do they compare in terms of patient outcomes and recovery times to traditional open-heart surgery?

16 May 2024 3,336 2 View

What would be some simple definitions to give to coaches and athletes on force platform metrics?

Jump Momentum Force at min Displacement Avg Propulsive Force Braking RFD Avg Braking Force

12 May 2024 768 0 View

¿Como puedo transformar una variable donde predominan los ceros y también hay valores altos dispersos?

Tengo una columna de la variable de "precipitación" donde predominan los ceros y hay valores altos. No he podido transformarla para obtener una distribución normal, ya he usado métodos de log,...

05 April 2024 8,427 1 View

Is it necessary to include an FMO for 7-AAD dye in your Flow Cytometry experiment? If not, why?

I'm optimizing a flow cytometer experiment and one of my three stains is a 7-AAD Live/Dead stain. The other stains in my experiment are staining for CD34+ and CD45+ cells. Is it necessary to...

01 April 2024 3,060 0 View

Would you have a simple Protocol for Flow cytometry cross match test (in flow tubes)?

The cross-match test is an in vitro test to determine the presence of anti-lymphocyte antibody to donor cell antigens (lymphocytotoxic antibody) in serum of an individual with preformed...

01 April 2024 3,203 1 View

Buen día: Me interesa el tema del pensamiento no occidental ¿si alguién fuera tan amable de hacerme alguna sugerencia?

Buen día: Mi nombre es Uriel David Avilés Rangel, estoy interesado en el pensamiento no occidental desde la historia antigüa. Actualmente trabajo la laicidad de la Educación Superior en México,...

25 March 2024 6,179 1 View

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