I have that doubt. But I can´t find the aswer. Hope you know it! Greetings.
It is normal that isolates of the same strain transformed with different plasmids have difference in growth rate.
Looks like that you are doing viral induced gene silencing (VIGS) experiment. Seems that the two binary vectors (pTRV1 and pTRV2) contain two major different genes, pTRV1 contains RdRP (RNA depended RNA polymerase) and pTRV2 contains a 'target gene' (if you insert a gene in it) you are aiming to silence in the plants (see attached maps). These genes might cause the different growth rates of Agrobacterium hosting them.
That is right, Yuan-Yeu Yau. Both vectors carried different insertions and that may cause to perform differently during growth.
Interesting questions and scholarly reasoning from colleagues. I'm following.
Except mentioned two genes in my previous answer, the vectors also contain 'MP' (Movement Protein) and 'CP' (Coat Protein) of virus particle in pTRV-1 and pTRV-2 respectively (see previous attachment). Both of the constructs are also driven by 2x35S promoter, which is active in Agrobacterium. Therefore, these genes can be expressed in Agrobacterium and affact the growth rate of Agrobacterium cells.
Dear Omar Adbeel Avilés Osuna,
If your are using TRV-based binary vectors (pTRV-1 and pTRV-2) for your virus-induced gene silencing (VIGS) research, I found the attached paper is very useful, in terms of technique. They even included information of what 'size' of the targeted endogeous gene should be used (see below). I think that is an important piece of info to know. And, criticle for the experiment to work.
"Gene fragment chosen for silencing: Fragments of 300-1500 bp have been used successfully to induce VIGS. Silencing efficiency is reduced below 300 bp, whereas with longer fragments there is a strong possibility of the insert being lost from the recombinant virus."
Dear Yuan-Yeu Yau
Thanks for the paper, it is very useful !
Have a great day.
Who are the vendors to get empty vectors? I could not find info on invitrogen or addgene.
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