How to skip/escape transcription from an expression cassette?

Previously, I had read a mechanism about conditionally-skip transcription from a cloned cassette in an expression vector. But i can't find that paper. It was containing a cis-regulatory element as...

20 March 2020 3,289 3 View

Does alternative genetic code has an impact on the translated amino acid?

Most amino acids are encoded by more than one genetic codon; for example Alanine amino acid is encoded by four genetic codons (GCA, GCT, GCC and GCG). The question is; If you are cloning a protein...

01 February 2019 408 1 View

How to clone a RT-PCR-amplified gene into a plasmid?

In order to clone a gene (which is RT-PCR-amplified) into a plasmid and translated into its corresponding protein, Should the gene include a Kozak consensus sequence before the starting...

01 February 2019 7,508 7 View

In Molecularly Imprinted Polymers(MIPs) formulated by precipitation method, why does the prepared polymer is an agglomerate not a cloudy solution?

I used 1 mmol of the template, 3 mmol MAA and 20 mmol of EGDMA. First I tried in 30 ml of ACN and DMSO, it formed a very thick gel after 20 hr polymerization in 60 C. Then I used same mixture but...

06 July 2017 9,984 25 View

In Molecularly Imprinted Polymers(MIPs) formulated by precipitation method, how does the prepared polymer look like at the end of the polymerization?

10 November 2015 4,833 8 View

Can an antifungal (candida albicans) susceptibility test be performed on Sabouraud media?

Via disk diffusion method, I know it isn't the best media but could it be done? And would it be reproducible?

05 June 2014 278 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View