06 June 2018 1 4K Report

The title of paper on researchgate is not correct. You can check the journal publication. How to edit it?

More Basma El-Awady's questions See All
How to engineer a genetic code to be translated starting with an amino acid other than methionine?

Any suggestions for cloning a protein in an expression vector where the translated protein is starting with an amino acid other than methionine? If it is not possible, any suggestion for a...

18 February 2021 8,812 6 View

How to skip/escape transcription from an expression cassette?

Previously, I had read a mechanism about conditionally-skip transcription from a cloned cassette in an expression vector. But i can't find that paper. It was containing a cis-regulatory element as...

20 March 2020 3,409 3 View

Does alternative genetic code has an impact on the translated amino acid?

Most amino acids are encoded by more than one genetic codon; for example Alanine amino acid is encoded by four genetic codons (GCA, GCT, GCC and GCG). The question is; If you are cloning a protein...

01 February 2019 546 1 View

How to clone a RT-PCR-amplified gene into a plasmid?

In order to clone a gene (which is RT-PCR-amplified) into a plasmid and translated into its corresponding protein, Should the gene include a Kozak consensus sequence before the starting...

01 February 2019 7,652 7 View

In Molecularly Imprinted Polymers(MIPs) formulated by precipitation method, why does the prepared polymer is an agglomerate not a cloudy solution?

I used 1 mmol of the template, 3 mmol MAA and 20 mmol of EGDMA. First I tried in 30 ml of ACN and DMSO, it formed a very thick gel after 20 hr polymerization in 60 C. Then I used same mixture but...

06 July 2017 10,054 25 View

In Molecularly Imprinted Polymers(MIPs) formulated by precipitation method, how does the prepared polymer look like at the end of the polymerization?

Is it dispersed in the porogen used or precipitated as a powder? When I prepare it, the prepared polymer precipitate in the bottom of the flask with a light rubbery consistency? when it dries out...

10 November 2015 4,910 8 View

Can an antifungal (candida albicans) susceptibility test be performed on Sabouraud media?

Via disk diffusion method, I know it isn't the best media but could it be done? And would it be reproducible?

05 June 2014 357 5 View

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