Candida albicans is implicated in many biomaterial-related infections.Its in vitro antifungal susceptibility test is possible when C. albicans strains grown as biofilms against amphotericin B and fluconazole .Antifungal susceptibility tests for amphotericin B (Bristol-Myers Squibb), fluconazole (Pfizer Pharmaceuticals Group) and caspofungin (Merck Sharpe & Dome) are performed using the broth microdilution method according to CLSI M27A2 (25). The antifungal agents were obtained as standard powders and stock solutions were dissolved in water (caspofungin and fluconazole) or 100% dimethyl sulfoxide (amphotericin B). The final concentrations of the antifungal agents can kept in a rnage 0.03 to 16 µg/ml for caspofungin and amphotericin B and 0.125 to 64 µg/ml for fluconazole.