I used 1 mmol of the template, 3 mmol MAA and 20 mmol of EGDMA. First I tried in 30 ml of ACN and DMSO, it formed a very thick gel after 20 hr polymerization in 60 C. Then I used same mixture but in 60 ml of porogen, it form a lighter gel.. I used 90 ml of the porogen and i had the same result. finally I used 200 ml porogen but the media was too diluted for the polymer to form. I couldn't get the spericals MIP as mention in many journals.
Why does it form that gel/ agglomerate or slurry ? and What should I do to get the spherical MIP in large amount so no need to crushed it? How much is the solvent needed to form spherical MIP?
Can a surfactant help to solve this aggregation problem?
NB. after drying, the produced polymer doesn't give mono-disperse particles in aqueous media, I can still see clumps. However when dispersed in Chloroform and DCM I get a cloudy dispersion without visible particles.
The problem is DMSO!
DMSO is a good solvent for the polymer and attempts to keep the final polymer in the liquid phase. But as the polymer is crosslinked by EGDMA, it can't totally dissolve it but makes it jelly.
So, I suggest using another porogenic solvent instead of DMSO. In case there is solubility problem with the template and you have to use DMSO, try to decrease the DMSO/ACN ratio as much as possible.
BTW, for precipitation polymerization, keep the total monomers/solvent ratio around 2:98
Have you try with toluene or any other solvent? It is better if you try using toluene or acetonitrile. However, it still depends on the dissolution of functional monomer you used.
I used ACN and DMSO in a ratio (5:1) to be able to dissolve the drug. I've also maintained the total monomers/solvent ratio around 2:98.
I didn't use toluene because of safety issues.
I've also tried to dissolve the produced polymer but it was insoluble in most solvents. However, I t is well dispersed in chloroform and Dichloromethane
Did you achieve dispersed and non-aggregated particles as a product? the thing you wanted.
after 20 hrs of polymerization It was aggregated. After I completed the washing step, i dispersed them in a mixture of chloroform and dichloromethane and they were dispersed. However, when I tried to suspend them in ultra-pure water or PBS they are still aggregated
Hello Basma
You can find helpful references on attachment 1
https://www.undergraduatelibrary.org/system/files/researchprojectundergradfinal.pdf
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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwiso7WvlabWAhXFKsAKHUSlDycQFgg3MAQ&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC3189760%2F&usg=AFQjCNEnn_RUKi2NmOxTxXbHFtJatJoi5A
4
https://www.undergraduatelibrary.org/system/files/researchprojectundergradfinal.pdf
7
https://www.science.gov/topicpages/p/polymers+synthesis+characterization.html
13
https://digibuo.uniovi.es/dspace/bitstream/10651/30752/1/TD_GaberHashem.pdf
15
https://pdfs.semanticscholar.org/ed87/e2fd4e12c9c586c31de29d125f3ac5907b72.pdf
17
https://www.agilent.com/cs/library/primers/Public/5991-3326EN_SPHB.pdf
20
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=24&cad=rja&uact=8&ved=0ahUKEwi0v7eSl6bWAhVeGsAKHQDsB_E4FBAWCDEwAw&url=https%3A%2F%2Fec.europa.eu%2Fresearch%2Findustrial_technologies%2Fpdf%2Fnano-hands-on-activities_en.pdf&usg=AFQjCNEKOfHvzp2255NjEl0RO21aIjciGA
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