22 January 2020 3 501 Report

I am wanting to knock down and do combinations of knock downs via siRNA or possibly siRNA/shRNA combinations to look at the effects of the knock down of a specific gene family with 3 family members in MDA-231 breast cancer cells.

More Skylar King's questions See All
Plant extracts versus a fungus, why lower concentrations are sometimes more effective than the higher concentrations?

We conducted a laboratory experiment (two trials) on the effect of plant extract against rice blast fungus. The study involved 0, 25, 50, 75, and 100% concentrations of ethanolic extracts against...

13 October 2020 7,214 4 View

How to choose the right polymer that can disperse my nanoparticle properly?

I have copper nanoclusters, can you provide me guidelines on how to find an appropriate polymer that can disperse it properly?

13 May 2020 3,249 9 View

Why my restiction enzyme treated chromasomal DNA has Plasmid results?

I conducted a practical whereby previously isolated plasmid and chromasomal DNAs that had been electrophoriesd in sepeerate gells where then both treated with the same restiction enzyme (EcoR1)...

11 September 2019 8,730 3 View

Quantitative methods for perception analysis - is this possible?

Hi there, I am looking at the perceptions Key Stage 2 learners (Years 5 and 6) have towards iPads in the classroom. Is it possible to look at this question from purely a quantitative point of...

09 March 2019 9,892 1 View

Can anyone help me convert the concentration of human anti-hepatitis B antibody (Hepabig) from IU/ml to mg/ml?

I am using Hepabig brand which only states 100 IU/0.5 ml concentration of antibody but no mg/ml immunoglobulin concentration estimate.

05 March 2019 5,808 15 View

Increasing solubility of 20-50 nm diameter Carboxylate modified Polystyrene Nanoparticles?

We want to inject these into a living insect. They come in a solution that includes a surfactant that will harm/kill the insect after injection and they appear to aggregate heavily when the...

12 February 2019 9,892 2 View

Why is mass spectrometry not inherently quantitative?

I am going to be working with a proteomics core to look at phosphorylation of signaling intermediates in response to a hormone of interest. I want to have a better understanding of mass...

02 September 2018 1,692 16 View

I'm characterizing the lipids produced by E. Coli under different temperature conditions could you suggest a TLC or GC protocol w/ standardization?

I am working on the characterization of the lipids produced by E.coli (ATCC 25922) under different temperature conditions for their eventual use in a leakage assay. Could anyone suggest a...

06 February 2018 2,671 4 View

Foaming is typically considered a problem in bioreactors. Does anyone purposefully grow microbes in a pure foam reaction?

Foaming is typically considered a problem in bioreactors. Does anyone purposefully grow microbes in a pure foam reaction? Does anyone induce foam formation in a bioreactor as a way of speeding...

03 December 2017 1,219 1 View

Clinical trial sample size calculations?

I am just becoming familiar with the sample size calculation for clinical trials The questions below are conceptual (not on actual calculations) 1. If an intervention is expected to influence...

18 October 2016 3,580 7 View

Similar questions and discussions
Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

It is easier to knockdown a gene than overexpress a gene when generate a stable cell line?

I am just so confused that some people tell me knockdown is easier than overexpression when generating a stable cell line. I think it depends on the gene. Cells won't be happy whether we try to...

15 February 2021 1,370 2 View

SiRNA transfection efficency and results interpretation ?

i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...

11 February 2021 8,145 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

Measuring small RNA abundance, without sequencing?

Dear all, Do you know any reliable and fast method to measure the levels of plant small RNAs (21,22 and 24 mer)? I am interested in only comparing the abundance of those small RNA between mock...

21 January 2021 4,002 6 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

What will be the life of siRNA (liquid form) at -20 degree, if kept unopened?

Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...

10 January 2021 6,346 6 View

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