Can I do double or triple siRNA knockdown in MDA-231 breast cancer cells?

More Skylar King's questions See All

Can we find the Gromacs version from XTC file or TRR?

Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...

16 July 2024 7,205 2 View

How to correct my name?

How can I correct my name on my profile?

30 June 2024 10,166 1 View

How to actually contact Research Gate to discuss bogus claim of copyright infringement?

For this research and its publication, King, A.J., J.K. Griffin and F. Roslan. 2014. In vitro and in vivo addition of dried olive extract to poultry products. J. Agric. Food Chem., 2014, 62 (31),...

04 March 2024 6,508 2 View

Cadence generic PDK FinFet download?

I have managed to Cadence generic PDK 45nm from the web [1] and found it very good. If somebody could send or link the Generic cadence FinFet PDK "cds_ff_mpt" for studying that'd be great. [1] I...

29 February 2024 5,561 1 View

Primates respond to the sudden appearance of a predator in their midst with random motion. Do humans do something similar?

Itani distinguished between the exterior and interior predator. Altmann and Altmann described an incident in which a leopard jumped from bushes into the midst of a baboon group and the baboons...

15 February 2024 1,896 1 View

Gaussian Connectivity Error?

Hi! I am working on a thesis project using Gaussian to model reactivtiy in compounds and I keep getting the error message " End of file reading connectivity." I have looked through Gaussian...

03 February 2024 5,869 2 View

Why school should not mandate student to wear uniform?

1.good impact of it 2.how if allow to wear sport uniforms everyday. 3.i need some report and some case about it

09 October 2023 8,417 1 View

Can anyone recommend resources and assessments for binge eating in children?

Female child age 9 - approaches to ongoing work and assessment sought

18 May 2023 9,163 0 View

Searching for robot-assisted stereotaxic protocol?

Hello, My lab recently purchased a robot stereotaxic instrument for performing IC surgeries on mice. https://robot-stereotaxic.com/motorized-stereotaxic-instrument We're in the process of...

01 March 2023 1,859 4 View

Protocol or tips for using Stereotaxic robotic system for murine IC surgery?

Hello, my lab recently purchased a robot stereotaxic instrument for performing IC surgeries on mice. We're in the process of learning how to use it, but was curious to know other people's...

08 February 2023 6,707 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

Is antibiotic bad for transfection?

I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...

07 July 2024 8,671 6 View

Reliable transfection method for Jurkat cells?

Hi to everyone! Does any of you have experience in transfecting siRNA in Jurkat cells and can provide a reliable method? I have both Neon transfection system and RNAiMAX Lipo, but to date none of...

30 June 2024 6,901 4 View

How to avoid errors while doing qpcr?

I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...

07 June 2024 7,292 1 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

My transduced cell can resist Puromycin and 99% express GFP, BUT can not show me the difference in the knockdown. what could be the possible reason?

My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?

31 May 2024 454 2 View

Is there any sequence that needs to be added when synthesizing and cloning shRNA?

To create a cell line overexpressing a specific microRNA, I'm planning to synthesize and clone the shRNA sequence into a vector. Would it be sufficient to add only the termination sequence (TTTTT)...

27 May 2024 4,206 0 View

Saurabh Mandal

It is possible to do multiple knowdown of genes of the same family. MDA-MB-231 itself a very robust cell line. Kindly find the attached papers.

Article Triple Combination of siRNAs Targeting TGF beta 1, TGF beta ...

Article Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2,...

Article Combination of siRNA-directed Gene Silencing With Cisplatin ...

Regards

Saurabh

Andreas Eisenreich

Sure. The most important point is the correct concenentration of si/shRNA in your experiments.

Shang Fei-Fei

Yes, you can try it. siRNAs have to be very specific. And you also have to find the right concentration.