12 November 2019 1 5K Report

Or should I use RNA polymerase and Reverse transcriptase to make RNA Spinach aptamers?

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What are the applicable reinforcement learning based control methods for systems that have control delay?

I am studying the experimental application of reinforcement learning (RL) based control methods. For that reason, I modeled a nonlinear system without delay and trained the RL agent. In this...

02 February 2021 7,999 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

How can I produce Alpha-monopropionin and Alpha-monobutyrin in lab scale?

I understand that glycerol should be reacted with propaoiocic acid to produce monopropionin and with butyric acid to produce monobutyrin , if you can help with the reaction conditions based on 1...

13 January 2021 1,151 4 View

What is the appropriate period for indexing an article in the Scopus database?

Dear RG members The indexing of a published article in the Scopus database may depend on several factors. The issue is that my colleague and I have published separately two articles in the same...

16 December 2020 4,361 19 View

I need equations to calculate the activity of the enzyme glutamanase and basic phosphatase in soil and plants?

I need the rates for calculating the mentioned enzymes after the laboratory work. I have trouble calculating and extracting the activity of the mentioned enzymes in soil and plants because of the...

03 November 2020 8,601 1 View

How can I know that a variant is novel?

I found a variant which is not reported in gnomAD or ExAC, but still has an rs. Should I consider it as a novel variant? when can I say that ceratin variant is novel?

15 October 2020 9,539 3 View

Do you have any qualitative or quantitative methodologies for evaluating gender equality plans in HE?

I am collating evaluation methodologies. So, broader methodologies for inclusion in my report are also welcome. Please also indicate the preferred citation for the evaluation methodology.

14 October 2020 4,266 7 View

How to compare the effect of changing single amino acid in a protein in a 3D model structure using Swiss-Model?

I run sequening on a sample and found a pathogenic variant. Then I draw a 3Dprotein model on Swiss-Model for the wild type protein and the mutatnt protein. I don't know how to comment on things...

05 October 2020 579 4 View

What are the recent research direction of Skin Cancer Detection ?

I am doing a research on Skin Cancer Detection. I have read a lot of redundant research papers about the problems which related to the same topic. Therefore, I wish to know the recent research...

04 October 2020 8,628 1 View

What can I refer for Depth estimation using multiple camera input?

Currently, I am working on my final year project which involves depth estimation form multiple camera inputs for video analysis purposes. I am new in this field and struggling to look a the...

30 September 2020 3,642 3 View

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Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

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Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

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How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

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So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

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Why might my GFP tag be dissociating from my plasmid after transfection?

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Which fluorescent dye to use for measuring total ROS production?

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