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Questions related from Mustafa Divyapıcıgil
It was left out for overnight in room temperature. Can we use it for experiments or is it expired? SDS says it should be stored in -20. Has anyone had the same issue and tried to use it?
02 March 2022 9,403 1 View
I need to store my extra carvedilol. However, I could not find this information in literature. Could you help me? Thank you.
16 June 2021 8,074 3 View
I am using two inverse PCR primers to add a sequence into my plasmid. After PCR I am phosphorylating and ligating it. Also, I am using bacterial transformation to antibiotics resistance check of...
12 March 2020 8,342 3 View
Or should I use RNA polymerase and Reverse transcriptase to make RNA Spinach aptamers?
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I am currently producing a recombinant protein via X-33 Pichia pastoris but production level is not enough. I've used Pgapz alpha a as the vector and YEPD as the medium. I'm using 10 ml YEPD for...
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How can i elute His-Tagged proteins via using ELISA instead of IMAC? If your suggestion would be pH alteration, would it be a trustable way?
11 August 2016 8,075 8 View
I need a free web based or windows software based bioinformatics tool that can design an antibody against given antigen sequence. I have found Abie Pro 3.0 but i could not understood how to use it.
24 July 2016 6,302 3 View
