Is it possible to do a (moderation) subsample analysis when both the IDV and Moderator are dichotomous variables?

At the moment I am writing my Thesis. I want to test the moderating effect of industry relatedness on a divestiture - firm performance relationship using subsample analysis. However, when...

28 April 2019 6,100 4 View

Cloning: BP reaction of large fragment: only a smaller fragment is found?

I perform long range-PCR of a 9kb fragment and purify the 9kb band from a gel. However after doing multiple BP reactions, i keep getting a smaller 3kb fragment that is in my plasmid, and no 9kb...

29 November 2017 8,621 3 View

Multiple bands after RNA transcription of a large gene?

I see multiple bands after performing transcription of a large gene and letting ut run over a denaturing RNA gel. Does anyone know possible causes and how to get rid of the unwanted smaller bands?

07 March 2017 4,340 3 View

Help on Gateway Cloning a large PCR fragment (10kb)?

12 December 2016 4,933 3 View

Does the RNeasy mini kit allow for RNA extraction of large fragments (>10kb)?

15 November 2016 2,414 5 View

Does anyone have a good protocol for obtaining large cDNA fragments (>10kb) through RT-PCR?

15 November 2016 4,714 3 View

What are normal water soluble concentrations of Iron and Aluminium in (calcium rich) soils?

I measured total Iron and Aluminum in water extracts of soil samples (1:10) by an IPC-OES. For our soil quality analysis/index method we like to use, these elements turn out to be incorporated for...

18 May 2016 654 7 View

Can anyone specify the value of interest of binary dependent variable in glmer() formula?

10 February 2015 2,559 2 View

Difference between 2 Cu xrd spectra

The xrd spectra are from cu particles . In the first xrd spectrum 2 more peaks are shown while in the second xrd spectrum none of these peaks are shown. The first spectrum comes from Cu particles...

01 January 1970 2,101 3 View

Is there a complete phylogenetic description of the Solanaceae family?

01 January 1970 2,894 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Are there any known solvothermal methods to synthesize MoS2 nanosheets using solvents other than water (like ethanol/ethanol-water mixture) ?

I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.

22 February 2021 9,246 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View