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Questions related from Jeroen Vierstraete
I perform long range-PCR of a 9kb fragment and purify the 9kb band from a gel. However after doing multiple BP reactions, i keep getting a smaller 3kb fragment that is in my plasmid, and no 9kb...
30 November 2017 8,695 3 View
I am wondering if cleaning my bench with ethanol is sufficient to remove RNase activity. Or should I use products like RNase ZAP?
05 October 2017 2,108 1 View
I see multiple bands after performing transcription of a large gene and letting ut run over a denaturing RNA gel. Does anyone know possible causes and how to get rid of the unwanted smaller bands?
08 March 2017 4,420 3 View
I am currently trying to Gateway clone a large PCR fragment. I have been able to PCR it perfectly and purify it through gel extraction. However, after BP reaction and transformation I have no...
13 December 2016 5,017 3 View
I want to amplify the cDNA sequence of a large gene in zebrafish (around 10kb). I am looking for any tips around RNA extraction protocol and cDNA synthesis so that my whole cDNA fragment isn't...
16 November 2016 4,800 3 View
Hello, I want to know if anyone has been able to get large RNA fragments from performing RNA extraction through the RNeasy mini kit. Many thanks! Jeroen
16 November 2016 2,498 5 View
Is it possible for me to freeze my product after performing a restriction digest so that I can continue working tomorrow? Or is it better to place the product at 4°C?
03 December 2015 8,077 11 View