I designed a pair primer when i blast in ncbi. blasting showed only in my microorganism it is enough for claiming specific a primer?
you can claim, but it is difficult due to chances of high rate of mutation rapidly in case of microorganism. Therefore, your primers reproducibility will be decreased rapidly, even you do the experiment by collecting the same microorganism outside of your lab.
If BLAST yields 100% coverage and 100% match just to your target then you can say with absolute confidence that your primer(s) is specific
Absolutely you can claim
If it is your design and match when you test it.
with reference to the sites used
It is very complicated to claim that your primer is specific for your microorganism. The problem is that how can you be sure that this primer can not work or identify all other microorganisms distributed in the universe. Many claimed primers designed for specific pathogenic bacteria are found to identify other non pathogenic microflora naturally found in soil.
The answer to your question, in such a case, you can claim that your designed primer is specific for targetting specific microorganisms in pure culture.
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