Can i claim my primer is specific for my microorganism?

I designed a pair primer when i blast in ncbi. blasting showed only in my microorganism it is enough for claiming specific a primer?

04 May 2019 3,722 6 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

11 December 2018 610 11 View

Is degenerate primers appropriate for real time pcr?

hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...

11 December 2018 8,600 2 View

Which band is appropriate for sequencing ?

10 November 2018 2,564 4 View

Which band is appropriate for sequencing ?

HI... i have some strain agrobacterium rhizogenes and I want to know variation between my straines i choiced a primer from article and i blast that primer in NCBI ,result blast is : product...

01 January 1970 9,833 4 View

Flanoeid extraction from hairy root

hi... I want to extract Flanoeid from hairy root but we need freeze-drier but my laboratory don"t have any freeze-drier... what is the instrument I can use instead of freeze-dried I can ? why we...

01 January 1970 4,991 2 View

Which sofware is appropriate for desiginig primer for real time?

Which sofware is appropriate for desiginig primer for real time? I want to design a primer for realtime PCR but I PREFER NOT TO USE DNase. my gene has interon region would u plz suggest an...

01 January 1970 9,125 1 View

Which sofware is appropriate for desiginig primer for real time?

I want to design a primer for realtime PCR but I PREFER NOT TO USE DNase. my gene has interon region would u plz suggest an appropriate software for this?

01 January 1970 1,883 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View