I want to design primer but my gene is partial cds i want to know how i can find all plant of asterase that have my gene
I designed a pair primer when i blast in ncbi. blasting showed only in my microorganism it is enough for claiming specific a primer?
04 May 2019 3,756 6 View
I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...
11 December 2018 643 11 View
hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...
11 December 2018 8,629 2 View
HI... i have some strain agrobacterium rhizogenes and I want to know variation between my straines i choiced a primer from article and i blast that primer in NCBI ,result blast is : product...
10 November 2018 2,592 4 View
01 January 1970 9,864 4 View
hi... I want to extract Flanoeid from hairy root but we need freeze-drier but my laboratory don"t have any freeze-drier... what is the instrument I can use instead of freeze-dried I can ? why we...
01 January 1970 5,024 2 View
Which sofware is appropriate for desiginig primer for real time? I want to design a primer for realtime PCR but I PREFER NOT TO USE DNase. my gene has interon region would u plz suggest an...
01 January 1970 9,153 1 View
I want to design a primer for realtime PCR but I PREFER NOT TO USE DNase. my gene has interon region would u plz suggest an appropriate software for this?
01 January 1970 1,915 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View