Can i claim my primer is specific for my microorganism?

I designed a pair primer when i blast in ncbi. blasting showed only in my microorganism it is enough for claiming specific a primer?

04 May 2019 3,722 6 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

11 December 2018 610 11 View

Is degenerate primers appropriate for real time pcr?

hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...

11 December 2018 8,600 2 View

Which band is appropriate for sequencing ?

10 November 2018 2,564 4 View

How do i find all of plant of asterase have cinnamyl alcohol dehydrogenase ?

I want to design primer but my gene is partial cds i want to know how i can find all plant of asterase that have my gene

10 November 2018 5,524 0 View

Which band is appropriate for sequencing ?

HI... i have some strain agrobacterium rhizogenes and I want to know variation between my straines i choiced a primer from article and i blast that primer in NCBI ,result blast is : product...

01 January 1970 9,833 4 View

Flanoeid extraction from hairy root

hi... I want to extract Flanoeid from hairy root but we need freeze-drier but my laboratory don"t have any freeze-drier... what is the instrument I can use instead of freeze-dried I can ? why we...

01 January 1970 4,991 2 View

Which sofware is appropriate for desiginig primer for real time?

Which sofware is appropriate for desiginig primer for real time? I want to design a primer for realtime PCR but I PREFER NOT TO USE DNase. my gene has interon region would u plz suggest an...

01 January 1970 9,125 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View