Hi I am using RNAse Inhibitors (applied biosystems), which are enzyme-based and are meant to add during cDNA synthesis phase as per its manufacturer policy (1ul to each 20 ul RT reaction).
But, can I also add it just after the RNA extraction into my RNA elutes (FFPE-based) in the same ratio and store that RNA at 4 C for some time (like 24 hours) before carry on the reverse transcription phase? Just to avoid any further RNA degradation of FFPE extractions...Any informative thoughts!!!!!