It is a known fact now that interactions between bacterial (normal flora) and colon cancer cells influence the transcription profile of host cells or enterocytes.
So, how can I avoid or minimize or cleanse the intestinal bacterial load of human colon biopsies stored in RNALater at the time of surgery, before proceeding to RNA extraction from them to avoid at maximum all the bacterial RNA burden during my extraction for the human RNA. RNALater is a well-known bacteriostatic but not the bactericidal. Is there any sound protocol exist to tackle this issue or this bacterial RNA load is insignificant for various gene expression analyses over such colon samples?
Though my primers are quite specific, but still I have concerns. Need some deep insights or explanation about this scenario.
Thanx...
I suppose its practically impossible to get rid of 100% of bacterial RNA by any kind of cleaning. If the primes are specific the bacterial RNA should not interfere with your analysis.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA