The frequency of distribution of CRISPR elements among Gram -ve and Gram +ve bacteria almost the same or more in G +ve than G -ve or vice versa?

11 November 2016 0 4K Report

Also what about CRISPR-Cas9 presence level in Atypical bacterial genera?

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What impact would be there on RNA-SEQ experiments if we do not use any RNA Spike-In controls (like ERCC or SIRVs)?

Hi everyone, if I do not use spike-in RNA isoform controls for a customized RNA sequencing experiment (not the whole transcriptome, just based on few specific genes we are interesting), so is...

01 February 2020 8,059 2 View

Can I add "enzyme-based RNAse Inhibitors" to the extracted FFPE RNA elutions to avoid any possible RNA degradation by RNAses?

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Is there any undesirable effect of bacterial RNA load of normal intestinal flora present on human colon samples for the gene expression analyses?

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09 October 2018 6,099 1 View

Can we pool together the same type of samples in qPCR for delta-delta ct method?

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05 June 2018 8,555 7 View

Does "No Primer Dimer Formation" guarantee the absolute utilization of entire primer content (F & R both) in a PCR reaction?

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10 November 2017 4,434 4 View

How Spermidine alleviates in vitro full-length cDNA synthesis from RNA?

Spermidine through which mechanism, behavior or pathway results in stable full-length cDNA from the RNA when is used with certain reverse transcriptases, like AMV in RT step. What is its actual...

10 November 2017 5,993 2 View

How to get rid of PCR amplification artifacts while using the Taq DNA polymerase in PCR to prepare the libraries for NGS applications?

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04 May 2017 4,099 4 View

How an Asymmetric qPCR (unequal forward and reverse primers) results in an improved Melt Curve?

A low concentration of a primer than the other one in high concentration or even in volume often exhibits good melt curve appearances. What could be the reasons behind this? Is this dependent on...

10 November 2016 4,951 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

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03 March 2021 1,682 3 View

Can we classify HV and LV lines based on line length?

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03 March 2021 4,116 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

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01 March 2021 9,701 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Distribution of particles in metal matrix?

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28 February 2021 7,397 8 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

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How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

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Compact antenna for the frequency range 1 MHz-5 MHz?

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What is the exact mechanism of the antimicrobial effect of local anesthetics?

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27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

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24 February 2021 9,839 3 View