Can I add "enzyme-based RNAse Inhibitors" to the extracted FFPE RNA elutions to avoid any possible RNA degradation by RNAses?

Hi I am using RNAse Inhibitors (applied biosystems), which are enzyme-based and are meant to add during cDNA synthesis phase as per its manufacturer policy (1ul to each 20 ul RT reaction). But,...

04 May 2019 9,014 2 View

Is there any undesirable effect of bacterial RNA load of normal intestinal flora present on human colon samples for the gene expression analyses?

It is a known fact now that interactions between bacterial (normal flora) and colon cancer cells influence the transcription profile of host cells or enterocytes. So, how can I avoid or minimize...

09 October 2018 6,099 1 View

Can we pool together the same type of samples in qPCR for delta-delta ct method?

Is it worthy to pool all the samples together belong to a same group, but originated from different animals of a same species, to save time and resources (reagents)? For example; From my negative...

05 June 2018 8,555 7 View

Does "No Primer Dimer Formation" guarantee the absolute utilization of entire primer content (F & R both) in a PCR reaction?

Dear all, in a PCR (conventional & realtime both), if we achieve our desired product (GOI) and along with, there is also no primer dimer (PD) formation seen on the gel and melt curve, does...

10 November 2017 4,434 4 View

How Spermidine alleviates in vitro full-length cDNA synthesis from RNA?

Spermidine through which mechanism, behavior or pathway results in stable full-length cDNA from the RNA when is used with certain reverse transcriptases, like AMV in RT step. What is its actual...

10 November 2017 5,993 2 View

How to get rid of PCR amplification artifacts while using the Taq DNA polymerase in PCR to prepare the libraries for NGS applications?

Taq DP is very well known to induce Indels and single base substitutions etc. So if any polymorphism or genetic variation detected later on, through any NGS platform, what makes us sure that these...

04 May 2017 4,099 4 View

How an Asymmetric qPCR (unequal forward and reverse primers) results in an improved Melt Curve?

A low concentration of a primer than the other one in high concentration or even in volume often exhibits good melt curve appearances. What could be the reasons behind this? Is this dependent on...

10 November 2016 4,951 3 View

The frequency of distribution of CRISPR elements among Gram -ve and Gram +ve bacteria almost the same or more in G +ve than G -ve or vice versa?

10 November 2016 4,244 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View