Can I add PFA to serum sample for viral inactivation ?

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What are gaps in diabetic wound healing techniques or dressings formation research?

Wound Healing research gaps using some polymers

28 July 2024 5,178 0 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Je veux des articles et des ouvrages en rapport avec mon sujet de recherche?

voilà les mots clés: la communication ,sage femme; technolecte

27 May 2024 1,740 1 View

Is there anyone can help me with finding some samples of source codes or designed projects for OMnet++, SUMO, VANET Routing Protocol ?

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19 May 2024 8,866 2 View

About subtypes of Dyslexia : Single article containing: "Double Deficit Dyslexia," "Surface Dyslexia", "Rapid Naming Deficit"...?

I am unable to find a source containing all subtypes of dyslexia ("Double Deficit Dyslexia," "Surface Dyslexia", "Rapid Naming Deficit"...) in a single article to use as a reference for my...

27 March 2024 8,454 2 View

RNA-seq kits for viruses without polyA tail?

Can anyone please suggest me some commercially available kits specifically designed for sequencing RNA viruses (in particular I am targeting a positive ssRNA virus that does not have a poly(A)...

18 March 2024 6,366 1 View

How to extract data from figures with high accuracy?

Such as studies that represent results (mean, standard deviation) with graphs rather than numerical data

08 March 2024 3,555 1 View

Apoptosis assay using flow cytometry, can I use annexin FITC in GFP fluorescent cells?

I have transfected cells with GFP, which emit in close range to FITC and used apoptosis kit that have annexin conjugated with FITC to measure apoptosis and PI to measure death. I am gating for GFP...

07 March 2024 9,673 5 View

How to recover single strand dna after AMpure XP Beads purification ?

Hello! I have a problem with my purification step. I have a single strand dna (after a digestion using Lambda Exonuclease) that I need to purify before injecting it as a dna donnor in C.elegans...

06 March 2024 5,529 0 View

Do you suggest that I order my kit from BT Lab?

I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.

27 February 2024 6,940 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Immunofluorescence staining for only plasma membrane proteins?

Hello! I am having trouble staining for plasma membrane localization of a transporter. This transporter also resides intracellularly, however, I am only interested in staining its plasma membrane...

28 May 2024 8,249 5 View

Are there people who success to staining pax7 on mouse tissue (frozen, 4%PFA)?

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27 May 2024 1,597 1 View

Recommendation for pretreatment of pup mouse liver for FFPE tissue?

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08 May 2024 8,349 1 View

What is the best approach for embedding and cryosectioning rodent optic nerves?

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28 April 2024 2,622 0 View

Immunohistochemistry staining coming out poorly following a bad perfusion. What is the cause?

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23 April 2024 4,160 1 View

Can anyone provide me with a suitable protocl for Nrf2 immunoflorescnce staining?

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16 April 2024 6,086 1 View

How to perform the embedding and cutting of 0,4 um transwell Pet inserts to visualize polarized cell layer for histology?

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16 April 2024 6,517 1 View

What is protocol ICC protocol for Caspase-3 (Apoptosis)?

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14 April 2024 1,160 3 View

Asma Ach

Thanks a lot Warren J Gallin for your very prompt and kind reply.

Do you recommend any other alternative/solution to inactivate the virus in serum? (the serum sample will be used for multiplex cytokine assay purposes).

Ute Neubacher

You can try NaN3 (Sodium Azide) in a concentration between 0,02 and 0,05%. This substance is used in antibody-solutions as a preservative. It protects antibody-solutions from microbiological growth.