I want to know about binding mechanism of Cas9 & gRNA
I think there are relationships between the cas9 and DNA .
Virus affect on DNA of bacteria; it will make some damage or change and prevent the virus from reading coding
I think that
I ran Process (Model 7) for my research and I found a significant interaction on the A path. I tested for the indirect effect and got a significant indirect effect, but the index of moderated...
15 June 2024 6,794 3 View
how can i have one peak in melting curve of primer in Real-time PCR?
06 May 2024 419 4 View
Hello Can anybody suggest me good C18 column for the separation of small molecules which has a long life?
06 February 2024 5,719 4 View
Hello, I have nylon fabric dyed with Acid dye. I need to prove for part of my project, is it possible or not possible, the reaction between dye molecules absorbed in nylon fabric with water...
01 February 2024 1,671 4 View
Hi all I’m working with RP HPLC With injecting the sample from the same vial on a 4.6x250mm column (C18, 5um, 1ml/min) for several injections I get varying peak integrity Is there a rational...
28 December 2023 9,239 12 View
I want to create this material at lab
02 August 2022 872 0 View
I am writing to ask for more information about bioinformatics ideas for a humanized antibody. A humanized antibody has been causing me to wonder what kind of bioinformatician analysis I can do....
04 October 2021 6,961 3 View
I an undertaking tube formation matrigel assay using HUVEC cells. I've tried using 24 well plate and the images looked great! However, due to the expense of using matrigel I tried with a 48 well...
29 May 2021 1,177 2 View
I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...
11 July 2020 7,299 11 View
Hi everyone I am looking for a precise and comprehensive source for protocols of laboratory techniques which are used in biotechnology or cell culture and related subjects .Do you know guys any...
21 May 2020 1,064 8 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View