Is GAPDH a suitable reference gene for qPCR in transfected cells?

I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...

11 July 2020 7,139 11 View

Would you please introduce me a functional and updated protocol's sources for biotechnology research ?

Hi everyone I am looking for a precise and comprehensive source for protocols of laboratory techniques which are used in biotechnology or cell culture and related subjects .Do you know guys any...

21 May 2020 889 8 View

Why is a DNA partial sequence (16s rRNA) placed in a separate branch in phylogenetic tree?

Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...

23 April 2020 9,203 8 View

What is the effect of surrounding medium, e.g; Acidic nature, on spectral variation of LSPR for the Au nanoparticles?

I want to know origin of spectral shift in my plasmonic sensor when medium becomes more and more acidic.

08 October 2019 4,247 3 View

What is the best protocol for operating protein SDS -PAGE and Western blot ? Is there any video to learn more about details and trouble shooting?

hello every one I am looking for a really precise protocol for doing protein SDS PAGE and also Western blot and of course a good source for how to deal with their trouble shooting . any kind of...

23 August 2019 2,104 5 View

How is stabilizing NeoRez R981 Polymer with Triethylamine?

I was reading the patent " Method of providing a substrate with a hydrophilic coating and substrates, particularly medical devices, provided with such coatings", and in one of the examples, it...

21 December 2018 4,397 3 View

What is the best source for learning Comparative Genomics ?

Hi I am looking for a really functional source for learning more about details on the topic of Comparative Genomics .I would be appreciated to introduce me couple of them .

16 November 2018 2,647 9 View

How to calculate concentration of drugs in small intestine?

Does anyone know if there is any formula I can use for calculation of a drug concentration (knowing oral dose) in small intestine and/or other parts of GI? Thanks

20 February 2018 2,352 1 View

I have synthesized ZnO particles with a unique morphology. How can I stimate the thickness of the particles?

It seems that these particles are formed in the shape of flakes with nanosize thickness.

15 May 2017 6,716 1 View

What is aripiprazol

Please tell me what is aripiprazol?

04 January 2012 7,098 15 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View