Can anybody suggest me good C18 column for the separation of small molecules which has a long life?

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Uddipta Ghosh Dastidar

Phenomenex Luna C18

Inertsil ODS 3V.

The life of a column depends a lot on proper regular washing, usage and maintenance though.

Srinivasan Jayakumar

Shimadzu Shim-pack GIST C18 Column

Agilent ZORBAX Eclipse Plus C18 Column

Waters ACQUITY UPLC BEH C18 Column

Saleem Naser

Ace C18 4.6x250mm

William Letter

Most any commercial HPLC column with a C18 phase would satisfy your request. A fresh, clean new column, which is sized correctly for your specific HPLC system, plumbing and application would be more important. Column "lifetime" is more a function of the specific application it is used for and the training of the user. No need to worry about such things for most samples. Instead, focus on learning the basics of HPLC (e.g. Column Void volume, K prime, proper washing/flushing etc) and training. Work with an experienced chromatographer to develop a method using standards at first. Determine the column's void volume, sample K prime and optimized wavelength (for UV/VIS samples).

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